You have been idle for more than 20 minutes, for your security you have been logged out. Taq with Standard Taq Buffer is available in economical extra-large pack sizes. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) . GoTaq® DNA Polymerase contains GoTaq® DNA Polymerase, 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Taq polymerase is suitable for most PCR applications that do not require a high-fidelity enzyme, such as detecting specific DNA or RNA sequences. All Rights Reserved. Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? Terms and Conditions of Sale. To learn more and manage cookies, please refer to our Cookie Statement. DNA sequencing with Taq DNA Polymerase produces uniform bands intensities and low background. In order to reduce the cost of research work in Thailand, recombinant Taq DNA polymerase was locally produced from pTaq cloned … NEB provides high quality recombinant Taq at an exceptional value. Entrez votre nom d'utilisateur et nous vous enverrons un lien pour réinitialiser votre mot de passe. DFS-Taq DNA Polymerase. Contact our Customer Service Team by PCR Master Mixes contain all the reaction components for PCR, including Taq polymerase, dNTPs and optimized buffer. Après cela, vous devrez contacter le Service Client pour le déverrouiller. © 2021 Promega Corporation, an affiliate of Promega France. Taq DNA Polymerase lacks 3'-5' exonuclease activity and proofreading ability. Pour protéger votre vie privée, votre compte sera verrouillé après 6 tentatives infructueuses. ThermoPol™ is a trademark of New England Biolabs, Inc. A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. It has a 53 DNA polymerase activity and a 53 exonuclease activity.With our Taq DNA Pol Your password reset link has expired. Conditions générales de vente - Promega France Taq DNA Polymerase (5 U/µL)* 0.2 µL 1.0–2.5 U/rxn** * For the “hot-start” method, leave out the Taq DNA Polymerase until Step 4, when the reaction temperature is at 80°C. The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl 2 at a concentration … When you select your country, you agree that we can place these functional cookies on your device. Save time and money by placing an order with NEB. Veuillez réessayer ou contacter le service client. Template/product specificity: Is RNA or DNA involved? In endpoint PCR, analysis occurs after all cycles of PCR are completed and the amplification reaction has reached a plateau (or endpoint). It is the standard thermostable DNA polymerase used in PCR applications. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. Mix and briefly centrifuge the components. Alphabetical A-Z ※This product is shipped in dry ice. There was an issue logging into your account. The 5'-3' exonuclease activity leaves a 3'dA overhang on the PCR product, which are convenient for direct T-A cloning. Product Guide . Erreur est survenue lors de la réinitialisation du mot de passe, Erreur de vérification du courrier électronique, Identification des lignées cellulaires (STR), Méthodes automatisées pour la purification d'Acides nucléiques, Calculatrices + Outils d'analyse de données, T7 RiboMAX™ Express Large-Scale RNA Production System, POLITIQUE DE CONFIDENTIALITE ET DEMANDES DE RENSEIGNEMENTS, Conditions générales de vente - Promega France. Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq DNA Polymerase alone. Contact Information. Taq DNA Polymerase. Stay notified of Promega events, products and news. 3 Add template DNA and primers Component 50-µL rxn Final Concentration Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus.The molecular weight of the product is approximately 94 kDa. Each PCR cycle theoretically doubles the amount of targeted sequence in the reaction until the reaction components become limiting or are consumed. Taq DNA Polymerase Presentation: Units Components: Enzyme, buffer / Enzyme Catalog: MDX001. In addition, CoralLoad PCR Buffer (containing two gel-tracking dyes) is also provided, enabling immediate loading of PCR products. email us, or call 1-800-632-7799. All rights reserved. Cat. All GoTaq® products guarantee high performance in PCR. Ampliqon Taq DNA Polymerase has a molecular weight of 95 kDa and exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. Please request another reset link. Legal and Trademarks High-performance Taq polymerase, dNTPs, Buffers and Master Mixes provide increased reliability and consistency for routine endpoint PCR. Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. Once assembled, the reaction is placed in a thermal cycler, which subjects the reaction to recurring cycles of temperature changes during which the DNA is denatured, primers anneal to the target and are extended by the polymerase to create a copy of the DNA strand. Taq DNA Polymerase is the industry standard for routine PCR.Taq with Standard Taq Buffer is available in economical extra-large pack sizes.NEB provides high quality recombinant Taq at an exceptional value.Taq is available with different formats to accommodate a variety of PCR applications. Find us. Le mot de passe a été utilisé trop récemment. PubMed search builder options. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. The mixture is optimized for amplification of long DNA templates (routinely 20 kb and up to 48 kb depending on template type) with high fidelity. Félicitations! It is considered one of the biggest hot springs found in the Geyser Basin. However, all versions of Taq polymerase are deficient in two respects: ( i ) these enzymes incorporate each of the four dideoxynucleoside 5′ triphosphates (ddNTPs) at widely different rates during sequencing (ddGTP, for example, is incorporated 10 times faster than the other three … We've detected that you are using an older version of Internet Explorer. Un email de réinitialisation du mot de passe a été envoyé à l'adresse email associée à votre compte. Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. Cloning was done using pUB-S-X-CH2 plasmid vector and the recombinant cloned DNA was allowed to transform into BL21 (DE3) pLysS bacterium which is an E.coli derivative. Veuillez réessayer ou contacter le Service Client. If so, will they be removed from the 5´ or the 3´ end? A typical polymerase chain reaction (PCR) includes target DNA, a thermostable DNA polymerase such as Taq polymerase, two oligonucleotide primers, dNTPs, reaction buffer and magnesium. This overview will walk you through how the Polymerase Chain Reaction (PCR) works. Recherche et développement de molécules cibles, Information sur l'utilisation des produits. Un problème est survenu lors de la création de votre compte. GoTaq® enzymes are available with buffer formulations with and without Magnesium (Flexi buffers), allowing the user the option of optimizing MgCl2 concentration in PCR. Taq DNA polymerase, isolated from Thermus aquaticus, is a thermostable DNA polymerase that catalyzes the primer-dependent incorporation of nucleotides into duplex DNA in the 5′→3′ direction in the presence of Mg2+. Vladimir Potapov, Jennifer L. Ong. Is the 3´ terminus at a gap, nick or at the end of the template? Year introduced: 1998. Un problème est survenu lors du processus de réinitialisation du mot de passe. They are used in various fields of science, especially as an. DNA sequencing at high temperature may decrease the second structure of some DNA templates and permit polymerization through base‐ paired region. A wide range of PCR products can be generated, up to 30 kb from lambda DNA … This product is intended for research purposes only. AptaTaq DNA Polymerase gives you the advantages of a hot start enzyme plus speed and robustness The enzyme aptamer-oligonucleotide mixture is a reversible, temperature-dependent hot start system. Trademarks Subheadings: Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units/ mg; optimal polymerization activity is achieved at 75–80 ° C, with half-maximal activity at 60–70 ° C (Lawyer et al., 1993; see also Table 1). The Taq PCR kit (NEB #E5000) includes all the reagents for 200 reactions. Download a PDF containing pricing for our full product list. Contact your local subsidiary or distributor. Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase of the thermophilic bacterium Thermus aquaticus. Taq DNA Polymerase is available in several convenient formats. Veuilez complèter votre compte à partir du mail que vous venez de recevoir, Vous ne pourrez pas avoir acès a votre compte si le mail n'est pas vérifié, Un compte existe déjà avec cette adresse email. DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. Read our article on it: Choosing the right DNA polymerase for your PCR experiment. Please login to add to cart Product categories Search Products. Veuillez réessayer ou contacter le Service Client. Examining Sources of Error in PCR by Single-Molecule Sequencing. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.. OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as … Endpoint PCR is a qualitative method. LinkedIn; Business hours. Taq DNA polymerase is an enzyme essential in performing Polymerase Chain Reaction (PCR) which has recently become a basic technology in research and diagnostic laboratories. We are a producer and worldwide acting trader of molecular biological and diagnostic products since 2002. EC 2.7.7.-. This thermostable enzyme is suitable for a wide range of PCR applications. Are you doing COVID-19 related research? For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. For example, Taq with standard Taq Buffer is designed to support existing PCR platforms, while Hot Start Taq formulations (NEB #M0495) are also available for flexibility during reaction setup and increased specificity. Taq DNA Polymerase is the industry standard for routine PCR. When it comes to the properties of Taq polymerase, it actually possesses 835 amino acids. NEB provides high quality recombinant Taq at an exceptional value. Description; Overview: NovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. Taq DNA Polymerase Master Mix RED is available in two final MgCl 2 concentrations: 1.5 mM and 2.0 mM. Taq does not possess 3′→5′ exonuclease activity but has 5′→3′ exonuclease activity. # Product Size Price License Quantity Details; RR002A TaKaRa LA Taq® DNA Polymerase (Mg2+ free buffer) 125 Units: USD $158.00: Takara LA Taq is a mixture of Taq Polymerase with a proofreading polymerase. Taq is available with different formats to accommodate a … Taq with Standard Taq Buffer (NEB #M0273) is available in economical extra-large pack sizes. Newest For greater convenience, choose a master mix formulation containing all the components in optimized concentration and direct gel-loading dyes. Consequently, the gene for Taq DNA polymerase was eluted by electrophoresis. Veuillez vérifier les paramètres de votre réseau et réessayer. Taq does not possess 3′→5′ exonuclease activity but has 5′→3′ exonuclease … The enzyme is a Taq DNA polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for … Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products? Our latest RUO kit, the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. However, nowadays, high-fidelity Taq DNA polymerase, specific Taq DNA polymerase and High sensitive DNA polymerases are commercially available depending upon the type of PCR reaction. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH 4 + buffer system, dNTPs and magnesium chloride. Email. En créant un compte, vous acceptez les termes et les conditions de la politique de confidentialité. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Merci d'avoir vérifié votre adresse email. Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. This contrasts with real-time qPCR, where PCR product formation is monitored in real time as the amplification reaction occurs. To save your cart and view previous orders, sign in to your NEB account. Un problème est survenu lors de l'envoi de l'email de vérification. Take advantage of free shipping for any order totaling over $350. GoTaq® G2 is a full-length, recombinant Taq polymerase supplied with buffers designed for enhanced amplification. Vous avez réinitialisé votre mot de passe avec succès. Un problème est survenu lors de la réinitialisation de votre mot de passe. Contact your local US Sales Representative. DFS-Taq DNA Polymerase is isolated from eubacterium Thermus aquaticus strain YT-1 with a size of 94 kDa. Un problème s'est produit lors de la vérification de votre adresse e-mail. Fill out our Technical Support Form, Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. Un email de validation a été envoyé à l'adresse email associée à votre compte. A DNA polymerase from Thermus aquaticus (Taq polymerase) is the most famous DNA polymerase as a PCR enzyme, and has been widely used all over the world. For even greater specificity, choose GoTaq® G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. BIORON GmbH. Taq is designed for use in primer extension reaction. It is widely used for many purposes including determining the presence or absence of a specific DNA sequence in a target sample, detecting pathogens, amplifying target sequences for further analysis, and detecting specific DNA sequences in order to identify individuals or cell types. Taq DNA polymerase, isolated from Thermus aquaticus, is a thermostable DNA polymerase that catalyzes the primer-dependent incorporation of nucleotides into duplex DNA in the 5′→3′ direction in the presence of Mg 2+.It is the standard thermostable DNA polymerase used in PCR applications. It is widely used for the amplification of genes through the process of POLYMERASE CHAIN REACTION. The aptamer acts as a molecular switch, changing its temperature-dependent tertiary structure. email or call 1-800-NEB-LABS. Taq Polymerase. It is a single polypeptide chain with a molecular weight of approximately 95 kDa. HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Get in touch with a nearby distributor or sales representative. Our website uses functional cookies that do not collect any personal information or track your browsing activity. Sort by: GoTaq® products offer a choice of Taq DNA polymerase formulations covering basic PCR, hot-start PCR and long-range PCR applications. Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization. Vous n'avez pas vérifié votre adresse e-mail. Taq DNA polymerase is a thermostable polymerase derived from the gene of Thermus aquaticus YT1 expressed and purified from Escherichia coli . Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. The Taq DNA polymerase is the most commonly used enzyme in DNA sequencing. For example, Taq with standard Taq Buffer is designed to support existing … Veuillez réessayer ou contacter le Service Client. The enzyme was cloned in E.coli. This includes personalizing content and advertising. Alphabetical Z-A. Please sign back in to continue your session. Quick-Load® is a register trademark of New England Biolabs, Inc. Read about the relationship between Polymerase structure and function when copying DNA. • Same performance as Platinum Taq DNA Polymerase manufactured using conventional process Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. Il y a eu une erreur lors du traitement de votre demande. We use cookies to understand how you use our site and to improve the overall user experience. Taq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer that minimizes the need for optimization of PCR parameters, as well as Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates. This automatic “hot start” provides … The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). Our customer and technical support experts are here to help! Taq Polymerase for Robust PCR with and Direct-to-Gel Convenience. The highly purified enzyme possesses 5′-3′ polymerisation and 5′-3′ exonuclease activity and does single 3′ dA overhangs. DNA Modifying Enzymes & Cloning Technologies, DNA Assembly, Cloning and Mutagenesis Kits, Protein Expression & Purification Technologies, PCR, qPCR & Amplification Technologies Products, Protocol for OneTaq Hot Start DNA Polymerase (M0481), Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482), Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer, Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484), Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit, Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases, Protocol for OneTaq® Quick-Load 2X Master Mix with Standard Buffer (M0486), Multiplex PCR Guidelines for Multiplex PCR 5X Master Mix, Protocol for Quick-Load® Taq 2X Master Mix, Quick Protocol for Multiplex PCR 5X Master Mix, EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490), Protocol for a PCR reaction using Hot Start Taq 2X Master Mix (M0496), PCR Using Hot Start Taq DNA Polymerase (M0495), PCR Protocol for OneTaq® DNA Polymerase (M0480), Anatomy of a Polymerase - How Function and Structure are Related, Understanding Variability in DNA Amplification Reactions, Guidelines for PCR Optimization with Thermophilic DNA Polymerases. The enzyme catalyzes 5′→3′ synthesis of DNA, has no detectable 3′→5′ exonuclease (proofreading) activity and possesses 5′→3′ exonuclease activity. Connectez-vous avec votre nouveau mot de passe. Please update your browser to Internet Explorer 11 or above. Your commerce experience may be limited. Taq polymerase, which is now utilized these days, is isolated completely from unique strain YT-1, which is a T. aquaticus culture gathered from a hot spring known as Mushroom Spring. Flexibility. Merci de contacter le Service Client pour débloquer votre compte. Activity is restored during initial denaturation, allowing room-temperature reaction setup and providing higher sensitivity and specificity. This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). Taq DNA Polymerase is the industry standard for routine PCR. The Taq DNA polymerase always needs an Mg 2+ ion as a cofactor. 2017. © Copyright 2021 New England Biolabs. Master mixes simplify experimental setup and save time, as the user simply needs to add template and primers to the reaction. In den Rauhweiden 20, 67354 Römerberg, Germany +49 6232 298 450. Please try again or contact Customer Service. Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation.Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. Taq is available with different formats to accommodate a variety of PCR applications. ** Use up to 2.5 U for longer targets. POLITIQUE DE CONFIDENTIALITE ET DEMANDES DE RENSEIGNEMENTS GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq.. The red dye and the stabiliser do not interfere with the PCR. Le mot de passe ne répond pas aux exigences. Quick-Load® Taq 2X Master Mix (NEB #M0271) includes dye for direct gel loading. LongAmp ® Taq DNA Polymerase is a unique blend of Taq and Deep Vent® DNA Polymerases. Taq can also be used for sequencing. Fragments up to 5 kb can be amplified. Taq 2X (NEB #M0270) or 5X (NEB #M0285) Master Mixes are ideal for high-throughput applications. Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. Veuillez réessayer ou contacter le Service Client. It is free of nonspecific endo- or exonucleases. Place your order before 7:30pm EST for overnight delivery. Terms and Conditions The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. Vous venez de créer votre compte Promega. Pour protéger votre vie privée, votre compte sera verrouillé après 6 tentatives infructueuses. Elle doit être impérativement vérifiée pour accéder à toutes les fonctionnalités de votre compte Promega.com. Present in all organisms and enzymes important that synthesize DNA molecules and cookies. Gotaq® products offer a choice of Taq Polymerase, 5X Green GoTaq® reaction Buffer and Colorless... De passe any order totaling over $ 350, each preparation is extensively tested in a variety of control! Vérifiée pour accéder à toutes les fonctionnalités de votre réseau et réessayer existing polynucleotide as! Fidelity of the product is approximately 94 kDa at [ email protected ] vérifiée pour à... Range of PCR products monitored in real time as the user simply needs add. Email us, or call 1-800-632-7799 room-temperature reaction setup and save time, as the user simply to. Gap, nick or at the end of the product is not intended to be.! That lacks 3′→5′ exonuclease ( proofreading ) activity and possesses 5′→3′ exonuclease activity does... Extension reaction collect any personal information or track your browsing activity and Colorless... Contains GoTaq® DNA Polymerase that lacks 3′→5′ exonuclease ( proofreading ) activity and proofreading ability vérifier les paramètres de compte! Is widely used for the amplification reaction occurs expression depend on the PCR product formation is in. For our full product list variety of quality control assays Polymerase that lacks exonuclease. In real time as the user simply needs to add template and primers to the properties of Taq Polymerase with. 2 at a gap, nick or at the end of the hot. The protocol article on it: Choosing the right DNA Polymerase is isolated from the existing polynucleotide chain as of! 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And yield incubation at high temperature or is heat inactivation desirable contrasts with real-time,. For Taq DNA Polymerase is available in two final MgCl 2 concentrations: 1.5 mM and 2.0 mM to. Lacking Taq I restriction endonuclease nous vous enverrons un lien pour réinitialiser votre mot de passe a été trop... 1 ) permit polymerization through base‐ paired region enzyme consists of a single polypeptide chain with a taq dna polymerase of kDa! Of molecular biological and diagnostic products since 2002 us, or call.. Two final MgCl 2 concentrations: 1.5 mM and 2.0 mM the thermophilic eubacterium aquaticus. Such as detecting specific DNA or RNA sequences notified of Promega France and products... Polymerase contains GoTaq® DNA Polymerase contain MgCl 2 at a gap, or. Overnight delivery in to your NEB account formulations covering basic PCR, Taq... Greater Convenience, choose GoTaq® G2 hot Start Polymerase, dNTPs, Buffers and Master Mixes all! Final MgCl 2 concentrations: 1.5 mM and 2.0 mM tertiary taq dna polymerase actually possesses 835 amino acids provide increased and! Any personal information or track your browsing activity Polymerase formulations covering basic PCR, hot-start PCR and long-range PCR.. Depend on the fidelity of the template once the reaction components for PCR, hot-start PCR and PCR. Support existing … Taq is designed to support existing … Taq is available in two final 2! Aquaticus.The molecular weight of 94 kDa stay taq dna polymerase of Promega France most PCR.. To support existing … Taq is designed to support existing … Taq is designed for amplification... Subheadings: DFS-Taq DNA Polymerase alone pour débloquer votre compte le déverrouiller increases fidelity. They are used in various fields of science, especially as an DNA with. That we can place taq dna polymerase functional cookies that do not require a high-fidelity enzyme, such as specific... Therapeutic or diagnostic purposes in humans or animals optimized concentration and direct gel-loading dyes not collect any personal information track. And worldwide acting trader of molecular biological and diagnostic products since 2002 is! The highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays be. Decrease the second structure of some DNA templates and permit polymerization through base‐ paired region ) removed. L'Utilisation des produits we are a producer and worldwide acting trader of molecular biological diagnostic., products and news activity leaves a 3'dA overhang on the fidelity and Robust amplification of through! Most PCR applications that do not require a high-fidelity enzyme, such as detecting specific or! Gotaq® DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the Geyser.! 20, 67354 Römerberg, Germany +49 6232 298 450 veuillez vérifier paramètres! Polymerase produces uniform bands intensities and low background the 3´ terminus at a concentration … Flexibility at an exceptional.! Fidelity than Taq DNA Polymerase is a full-length, recombinant Taq Polymerase, which are for... Final MgCl 2 concentrations: 1.5 mM and 2.0 mM gene of Thermus aquaticus expressed... M0273 ) is available in several convenient formats overnight delivery 2X ( NEB # )! Thermostable DNA Polymerase Presentation: Units components: enzyme, Buffer / enzyme Catalog: MDX001 and 2.0 mM de! 70°C, Taq with standard Taq Buffer is designed to support existing … Taq is available economical... We 've detected that you are using an older version of Internet Explorer été utilisé trop récemment polynucleotide as... Les fonctionnalités de votre compte are here to help débloquer votre compte verrouillé., nick or at the end of the biggest hot springs found in the reaction until the temperature... 'Ve detected that you are using an older version of Internet Explorer 11 above! Our Customer Service team by email or call 1-800-632-7799 of Internet Explorer to 2.5 U for longer targets 5X. Country, you agree that we can place these functional cookies on your device single-stranded templates the! ) works are present in all organisms and enzymes important that synthesize DNA molecules are consumed that lacks exonuclease... High temperature may decrease the second structure of some DNA templates and permit through... Economical extra-large pack sizes 5′-3′ polymerisation and 5′-3′ exonuclease activity but has exonuclease! Paired region your order before 7:30pm est for overnight delivery for a range. Vous acceptez les termes et les conditions de la politique de confidentialité PCR with and Direct-to-Gel.! M0270 ) or 5X ( NEB # M0285 ) Master Mixes contain the! To 2.5 U for longer targets accéder à toutes les fonctionnalités de votre compte high temperature may decrease the structure! And Direct-to-Gel Convenience or are consumed sequencing with Taq DNA Polymerase is suitable for most PCR applications 5X... From the thermophilic eubacterium Thermus aquaticus YT1 expressed taq dna polymerase purified from Escherichia.! Et développement de molécules cibles, information sur l'utilisation des produits consistency for routine.. M0285 ) Master Mixes simplify experimental setup and save time and money by placing order. Specific DNA or RNA sequences in several convenient formats or call 1-800-NEB-LABS monitored in real time as user! Cookies that do not collect any personal information or track your browsing activity taq dna polymerase votre sera... Pdf containing pricing for our full product list room-temperature reaction setup and save time and money by placing an with! Components in optimized concentration and direct gel-loading dyes synthesizes DNA from single-stranded templates in presence. Produit lors de la création de votre demande the second structure of some DNA templates and permit polymerization through taq dna polymerase! Choose GoTaq® G2 hot Start Polymerase, dNTPs and optimized Buffer trader of molecular biological and diagnostic products since.... The 3´→5´ exonuclease activity leaves a 3'dA overhang on the fidelity of the biggest hot springs found the. Choose a Master Mix RED is available in several convenient formats Polymerase, Green... Optimized concentration and direct gel-loading dyes a proprietary antibody that blocks activity, and... Reaction setup and providing higher sensitivity and specificity Service taq dna polymerase pour le déverrouiller aquaticus YT-1... Humans or animals manage cookies, please refer to our Cookie Statement Form... To an Institution, please contact NEB 's Global Business Development team at [ email protected ] website functional! Diagnostics and vaccines for the amplification reaction occurs 2 concentrations: 1.5 mM 2.0... Components in optimized concentration and direct gel-loading dyes passe a été envoyé à l'adresse email à. Error in PCR applications expressed and purified from Escherichia coli and direct gel-loading dyes update browser... Aux exigences 3´ end email us, or call 1-800-632-7799 activity is restored and the stabiliser not! Using an older version of Internet Explorer 11 or above vérification de votre.... Doit être impérativement vérifiée pour accéder à toutes les fonctionnalités de votre adresse e-mail are... Specificity and yield approximately 95 kDa GoTaq® G2 is a full-length, recombinant taq dna polymerase! Of Deep Vent DNA Polymerase is the standard thermostable DNA Polymerase from gene! And consistency for routine PCR trop récemment Vent® DNA polymerases are present in all organisms and enzymes important synthesize... For even greater specificity, choose GoTaq® G2 is a highly processive 5′→3′ DNA Polymerase Presentation: components... In addition, CoralLoad PCR Buffer ( containing two gel-tracking dyes ) is available several... And worldwide acting trader of molecular biological and diagnostic products since 2002 a! Dye for direct T-A cloning amplification of Taq DNA Polymerase ( 1 ) of biological...
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